BLASTP 2.0MP-WashU [12-Feb-2001] [linux-i686 01:36:08 31-Jan-2001]

Copyright (C) 1996-2000 Washington University, Saint Louis, Missouri USA.
All Rights Reserved.

Reference:  Gish, W. (1996-2000) http://blast.wustl.edu

Query=  gi|1786183|gb|AAC73113.1| (AE000111) aspartokinase I, homoserine
    dehydrogenase I [Escherichia coli]
        (820 letters)

Database:  ecoli.aa
           4289 sequences; 1,358,990 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done

                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

gb|AAC73113.1| (AE000111) aspartokinase I, homoserine deh...  4141  0.0       1
gb|AAC76922.1| (AE000468) aspartokinase II and homoserine...   844  3.1e-86   1
gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensi...   483  2.8e-47   1
gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia c...    97  0.0010    1



>gb|AAC73113.1| (AE000111) aspartokinase I, homoserine dehydrogenase I
            [Escherichia coli]
        Length = 820

 Score = 4141 (1462.8 bits), Expect = 0.0, P = 0.0
 Identities = 820/820 (100%), Positives = 820/820 (100%)

Query:     1 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60
             MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA
Sbjct:     1 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60

Query:    61 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120
             LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA
Sbjct:    61 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120

Query:   121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180
             ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP
Sbjct:   121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180

Query:   181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
             ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV
Sbjct:   181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240

Query:   241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
             PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD
Sbjct:   241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300

Query:   301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
             EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF
Sbjct:   301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360

Query:   361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420
             CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL
Sbjct:   361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420

Query:   421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL 480
             ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL
Sbjct:   421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL 480

Query:   481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540
             LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL
Sbjct:   481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540

Query:   541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600
             VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR
Sbjct:   541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600

Query:   601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660
             RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA
Sbjct:   601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660

Query:   661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720
             REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA
Sbjct:   661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720

Query:   721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780
             NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF
Sbjct:   721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780

Query:   781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820
             YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV
Sbjct:   781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820


>gb|AAC76922.1| (AE000468) aspartokinase II and homoserine dehydrogenase II
            [Escherichia coli]
        Length = 810

 Score = 844 (302.2 bits), Expect = 3.1e-86, P = 3.1e-86
 Identities = 222/705 (31%), Positives = 356/705 (50%)

Query:   116 DSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIA 175
             D++ A ++  GE  S  +M+ VL  +G     +D  E L A     +  VD   S   + 
Sbjct:   119 DAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE-RAAQPQVDEGLSYPLLQ 177

Query:   176 ASRI--PADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVY 233
                +  P   +V+  GF + N  GE V+LGRNGSDYSA  + A        IW+DV GVY
Sbjct:   178 QLLVQHPGKRLVV-TGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVY 236

Query:   234 TCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGT 293
             + DPR+V DA LL  +   EA EL+   A VLH RT+ P++  +I   ++ +  P    T
Sbjct:   237 SADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGST 296

Query:   294 LIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSS 353
              I           + +++ +++ +     P  +        +   + RA++  + +   +
Sbjct:   297 RIERVLASGT-GARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHN 355

Query:   354 SEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGIS 413
                 + FC        A + + E        GL   L + + LA++++VG G+ T   + 
Sbjct:   356 DRQLLQFCYTSEVADSALKILDEA-------GLPGELRLRQGLALVAMVGAGV-TRNPLH 407

Query:   414 A-KFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIG 472
               +F+  L    +      Q     S+  V+      + ++  HQ +F  ++ I + + G
Sbjct:   408 CHRFWQQLKGQPVEFTW--QSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFG 465

Query:   473 VGGVGGALLEQLKRQQSWLKNKH-IDLRVCGVANSKALLTNVHGLN----LENWQEELAQ 527
              G +G   LE   R+QS L  +   +  + GV +S+  L +  GL+    L  + +E  +
Sbjct:   466 KGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVE 525

Query:   528 AKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMD 587
               E      L   ++ +   + V++D T+SQ +ADQY DF   GFHV++ NK A  S  +
Sbjct:   526 QDEE----SLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSN 581

Query:   588 YYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKL 647
              Y Q+  A EK+ R +LY+  VGAGLP+   +++L+++GD ++  SGI SG+LS++F + 
Sbjct:   582 KYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQF 641

Query:   648 DEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPA 707
             D  + F+E    A + G TEPDPRDDLSG DV RKL+ILARE G  +E   + +E ++PA
Sbjct:   642 DGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPA 701

Query:   708 EFNAEGDVAAFMANLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDP 767
                  G +  F  N  +L++    R+  AR+ G VLRYV   D +G  RV +  V  + P
Sbjct:   702 HCEG-GSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHP 760

Query:   768 LFKVKNGENALAFYSHYYQPLPLVLRGYGAGNDVTAAGVFADLLR 812
             L  +   +N  A  S +Y+  PLV+RG GAG DVTA  + +D+ R
Sbjct:   761 LASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQSDINR 805

 Score = 321 (118.1 bits), Expect = 3.6e-27, P = 3.6e-27
 Identities = 108/406 (26%), Positives = 191/406 (47%)

Query:     5 KFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNI 64
             KFGG+S+A+ + +LRVA I+   ++   +  V+SA    TN L+  ++ + + + +   +
Sbjct:    16 KFGGSSLADVKCYLRVAGIMAEYSQPDDMM-VVSAAGSTTNQLINWLKLSQTDRLSAHQV 74

Query:    65 SDAERIF-AELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLH-GISLLGQCPDSINAAL 122
                 R +  +L++GL  A+    L  +  FV  +  ++  +L  GI+      D++ A +
Sbjct:    75 QQTLRRYQCDLISGLLPAEEADSL--ISAFVS-DLERLAALLDSGIN------DAVYAEV 125

Query:   123 ICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRI--P 180
             +  GE  S  +M+ VL  +G     +D  E L A     +  VD   S   +    +  P
Sbjct:   126 VGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE-RAAQPQVDEGLSYPLLQQLLVQHP 184

Query:   181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
                +V+  GF + N  GE V+LGRNGSDYSA  + A        IW+DV GVY+ DPR+V
Sbjct:   185 GKRLVV-TGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPRKV 243

Query:   241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
              DA LL  +   EA EL+   A VLH RT+ P++  +I   ++ +  P    T I     
Sbjct:   244 KDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLA 303

Query:   301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
                   + +++ +++ +     P  +        +   + RA++  + +   +    + F
Sbjct:   304 SGT-GARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQF 362

Query:   361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGM 406
             C        A + + E        GL   L + + LA++++VG G+
Sbjct:   363 CYTSEVADSALKILDEA-------GLPGELRLRQGLALVAMVGAGV 401


>gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensitive [Escherichia
            coli]
        Length = 449

 Score = 483 (175.1 bits), Expect = 2.8e-47, P = 2.8e-47
 Identities = 149/467 (31%), Positives = 233/467 (49%)

Query:     3 VLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQ---- 58
             V KFGGTSVA+ +   R ADI+ S+A    V  VLSA A ITN LVA+ E    G+    
Sbjct:     6 VSKFGGTSVADFDAMNRSADIVLSDANVRLV--VLSASAGITNLLVALAEGLEPGERFEK 63

Query:    59 -DALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDS 117
              DA+ NI      FA +L  L      +P   ++  +++    I  VL   + L   P +
Sbjct:    64 LDAIRNIQ-----FA-ILERLR-----YPNV-IREEIERLLENIT-VLAEAAALATSP-A 109

Query:   118 INAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVG-HYLESTVDIAESTRRIAA 176
             +   L+  GE MS  +   +L  R       D V K++     +  +  DIA      A 
Sbjct:   110 LTDELVSHGELMSTLLFVEILRERDVQAQWFD-VRKVMRTNDRFGRAEPDIAALAELAAL 168

Query:   177 SRIPA--DHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYT 234
               +P   + +V+  GF     KG    LGR GSDY+AA+LA  L A   +IWTDV G+YT
Sbjct:   169 QLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVDIWTDVPGIYT 228

Query:   235 CDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTL 294
              DPR V  A+ +  +++ EA E++ FGAKVLHP T+ P  +  IP  + ++ +P+A GTL
Sbjct:   229 TDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPRAGGTL 288

Query:   295 IGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSS 354
             +  ++ E+    + ++   N  + ++    M    G  A VF  ++R  ISV LIT  +S
Sbjct:   289 V-CNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLIT--TS 345

Query:   355 EYSISFCVPQSDCVRA-ERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGIS 413
             E S++  +  +      +  + +   +EL    L  + V E LA+++++G+ +    G+ 
Sbjct:   346 EVSVALTLDTTGSTSTGDTLLTQSLLMELSA--LCRVEVEEGLALVALIGNDLSKACGVG 403

Query:   414 AKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLF 460
              + F  L   NI +  I  G+S  ++  +V  +DA   V+  H  LF
Sbjct:   404 KEVFGVLEPFNIRM--ICYGASSHNLCFLVPGEDAEQVVQKLHSNLF 448


>gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia coli]
        Length = 241

 Score = 97 (39.2 bits), Expect = 0.0010, P = 0.0010
 Identities = 30/98 (30%), Positives = 45/98 (45%)

Query:   199 LVVLGRN-GSDYSAAVLAACLR-----ADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQ 252
             +V+L    G+ +     AACLR     AD     T VDGV+T DP + P A + + ++Y 
Sbjct:   131 VVILSAGTGNPFFTTDSAACLRGIEIEADVVLKATKVDGVFTADPAKDPTATMYEQLTYS 190

Query:   253 EAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQA 290
             E +E      KV+     T     ++P  + N   P A
Sbjct:   191 EVLEKEL---KVMDLAAFTLARDHKLPIRVFNMNKPGA 225


Parameters:
  E=0.01

  ctxfactor=1.00

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   +0      0   BLOSUM62        0.319   0.136   0.384    same    same    same
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +0      0      820       820     0.010  93 3  11 22  0.19    34
                                                    37  0.22    37


Statistics:

  Database:  /home/jes12/db/ecoli.aa
   Title:  ecoli.aa
   Posted:  2:52:35 PM EST Nov 18, 2001
   Created:  9:46:47 AM EST Nov 18, 2001
   Format:  XDF-1
   # of letters in database:  1,358,990
   # of sequences in database:  4289
   # of database sequences satisfying E:  4
  No. of states in DFA:  573 (61 KB)
  Total size of DFA:  281 KB (1149 KB)
  Time to generate neighborhood:  0.00u 0.02s 0.02t  Elapsed: 00:00:00
  No. of threads or processors used:  1
  Search cpu time:  1.58u 0.00s 1.58t  Elapsed: 00:00:01
  Total cpu time:  1.59u 0.02s 1.61t  Elapsed: 00:00:01
  Start:  Thu Dec  6 11:09:14 2001   End:  Thu Dec  6 11:09:15 2001
