BLASTP 2.1.3 [Apr-11-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|1786183|gb|AAC73113.1| (AE000111) aspartokinase I,
homoserine dehydrogenase I [Escherichia coli]
         (820 letters)

Database: ecoli.aa
           4289 sequences; 1,358,990 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAC73113.1| (AE000111) aspartokinase I, homoserine dehydrogen...  1567  0.0
gb|AAC76922.1| (AE000468) aspartokinase II and homoserine dehydr...   332  1e-91
gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensitive [E...   184  3e-47
gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia coli]          42  3e-04

>gb|AAC73113.1| (AE000111) aspartokinase I, homoserine dehydrogenase I [Escherichia
           coli]
          Length = 820

 Score = 1567 bits (4058), Expect = 0.0
 Identities = 806/820 (98%), Positives = 806/820 (98%)

Query: 1   MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60
           MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA
Sbjct: 1   MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60

Query: 61  LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120
           LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA
Sbjct: 61  LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120

Query: 121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180
           ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP
Sbjct: 121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180

Query: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
           ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV
Sbjct: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240

Query: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
           PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD
Sbjct: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300

Query: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
           EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF
Sbjct: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360

Query: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420
           CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL
Sbjct: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420

Query: 421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQXXXXXXXXXXXXXXAL 480
           ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQ              AL
Sbjct: 421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL 480

Query: 481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540
           LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL
Sbjct: 481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540

Query: 541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600
           VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR
Sbjct: 541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600

Query: 601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660
           RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA
Sbjct: 601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660

Query: 661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720
           REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA
Sbjct: 661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720

Query: 721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780
           NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF
Sbjct: 721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780

Query: 781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820
           YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV
Sbjct: 781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820


>gb|AAC76922.1| (AE000468) aspartokinase II and homoserine dehydrogenase II
           [Escherichia coli]
          Length = 810

 Score =  332 bits (850), Expect = 1e-91
 Identities = 243/821 (29%), Positives = 403/821 (48%), Gaps = 44/821 (5%)

Query: 5   KFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNI 64
           KFGG+S+A+ + +LRVA I+   ++   +  V+SA    TN L+  ++ + + + +   +
Sbjct: 16  KFGGSSLADVKCYLRVAGIMAEYSQPDDMM-VVSAAGSTTNQLINWLKLSQTDRLSAHQV 74

Query: 65  SDAERIF-AELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINAALI 123
               R +  +L++GL  A+    L  +  FV         +  GI+      D++ A ++
Sbjct: 75  QQTLRRYQCDLISGLLPAEEADSL--ISAFVSDLERLAALLDSGIN------DAVYAEVV 126

Query: 124 CRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIPADH 183
             GE  S  +M+ VL  +G     +D  E L A      +   + E        ++   H
Sbjct: 127 GHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAER---AAQPQVDEGLSYPLLQQLLVQH 183

Query: 184 ---MVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
               +++ GF + N  GE V+LGRNGSDYSA  + A        IW+DV GVY+ DPR+V
Sbjct: 184 PGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPRKV 243

Query: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
            DA LL  +   EA EL+   A VLH RT+ P++  +I   ++ +  P       G++R 
Sbjct: 244 KDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQ-----GSTRI 298

Query: 301 EDELP----VKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEY 356
           E  L      + +++ +++ +     P  +        +   + RA++  + +   +   
Sbjct: 299 ERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQ 358

Query: 357 SISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKF 416
            + FC        A + + E        GL   L + + LA++++VG G+        +F
Sbjct: 359 LLQFCYTSEVADSALKILDEA-------GLPGELRLRQGLALVAMVGAGVTRNPLHCHRF 411

Query: 417 FAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQXXXXXXXXXXXX 476
           +  L    +      Q     S+  V+      + ++  HQ +F  ++            
Sbjct: 412 WQQLKGQPVEFTW--QSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNI 469

Query: 477 XXALLEQLKRQQSWLKNKH-IDLRVCGVANSKALLTNVHGLN----LENWQEELAQAKEP 531
               LE   R+QS L  +   +  + GV +S+  L +  GL+    L  + +E  +  E 
Sbjct: 470 GSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEE 529

Query: 532 FNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQ 591
                L   ++ +   + V++D T+SQ +ADQY DF   GFHV++ NK A  S  + Y Q
Sbjct: 530 ----SLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQ 585

Query: 592 LRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGM 651
           +  A EK+ R +LY+  VGAGLP+   +++L+++GD ++  SGI SG+LS++F + D  +
Sbjct: 586 IHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSV 645

Query: 652 SFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNA 711
            F+E    A + G TEPDPRDDLSG DV RKL+ILARE G  +E   + +E ++PA    
Sbjct: 646 PFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEG 705

Query: 712 EGDVAAFMANLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKV 771
            G +  F  N  +L++    R+  AR+ G VLRYV   D +G  RV +  V  + PL  +
Sbjct: 706 -GSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASL 764

Query: 772 KNGENALAFYSHYYQPLPLVLRGYGAGNDVTAAGVFADLLR 812
              +N  A  S +Y+  PLV+RG GAG DVTA  + +D+ R
Sbjct: 765 LPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQSDINR 805


>gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensitive [Escherichia coli]
          Length = 449

 Score =  184 bits (467), Expect = 3e-47
 Identities = 142/471 (30%), Positives = 228/471 (48%), Gaps = 41/471 (8%)

Query: 3   VLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQ---- 58
           V KFGGTSVA+ +   R ADI+ S+A    V  VLSA A ITN LVA+ E    G+    
Sbjct: 6   VSKFGGTSVADFDAMNRSADIVLSDANVRLV--VLSASAGITNLLVALAEGLEPGERFEK 63

Query: 59  -DALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCP-- 115
            DA+ NI                    F + +   + +    +I+ +L  I++L +    
Sbjct: 64  LDAIRNIQ-------------------FAILERLRYPNVIREEIERLLENITVLAEAAAL 104

Query: 116 ---DSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTR 172
               ++   L+  GE MS  +   +L  R       D  + +     +  +  DIA    
Sbjct: 105 ATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIAALAE 164

Query: 173 RIAASRIPA--DHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVD 230
             A   +P   + +V+  GF     KG    LGR GSDY+AA+LA  L A   +IWTDV 
Sbjct: 165 LAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVDIWTDVP 224

Query: 231 GVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQA 290
           G+YT DPR V  A+ +  +++ EA E++ FGAKVLHP T+ P  +  IP  + ++ +P+A
Sbjct: 225 GIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPRA 284

Query: 291 PGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLIT 350
            GTL+  ++ E+    + ++   N  + ++    M    G  A VF  ++R  ISV LIT
Sbjct: 285 GGTLV-CNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLIT 343

Query: 351 QSSSEYSISFCVPQSDCV-RAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTL 409
             +SE S++  +  +      +  + +   +EL    L  + V E LA+++++G+ +   
Sbjct: 344 --TSEVSVALTLDTTGSTSTGDTLLTQSLLMEL--SALCRVEVEEGLALVALIGNDLSKA 399

Query: 410 RGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLF 460
            G+  + F  L   NI +  I  G+S  ++  +V  +DA   V+  H  LF
Sbjct: 400 CGVGKEVFGVLEPFNIRM--ICYGASSHNLCFLVPGEDAEQVVQKLHSNLF 448


>gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia coli]
          Length = 241

 Score = 41.6 bits (96), Expect = 3e-04
 Identities = 28/97 (28%), Positives = 44/97 (44%), Gaps = 8/97 (8%)

Query: 199 LVVLGRNGSDYSAAVLAACLR-----ADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQE 253
           +++    G+ +     AACLR     AD     T VDGV+T DP + P A + + ++Y E
Sbjct: 132 VILSAGTGNPFFTTDSAACLRGIEIEADVVLKATKVDGVFTADPAKDPTATMYEQLTYSE 191

Query: 254 AMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQA 290
            +E      KV+     T     ++P  + N   P A
Sbjct: 192 VLEKE---LKVMDLAAFTLARDHKLPIRVFNMNKPGA 225


  Database: ecoli.aa
    Posted date:  Dec 6, 2001  1:58 PM
  Number of letters in database: 1,358,990
  Number of sequences in database:  4289
  
Lambda     K      H
   0.319    0.135    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2022122
Number of Sequences: 4289
Number of extensions: 82424
Number of successful extensions: 256
Number of sequences better than 1.0e-03: 4
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 243
Number of HSP's gapped (non-prelim): 4
length of query: 820
length of database: 1,358,990
effective HSP length: 47
effective length of query: 773
effective length of database: 1,157,407
effective search space: 894675611
effective search space used: 894675611
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 92 (40.0 bits)
