ID   AF036895   standard; DNA; INV; 3693 BP.
XX
AC   AF036895;
XX
SV   AF036895.1
XX
DT   22-DEC-1997 (Rel. 54, Created)
DT   03-MAR-2000 (Rel. 62, Last updated, Version 6)
XX
DE   Chironomus thummi special lobe-specific silk protein ssp160 gene, complete
DE   cds.
XX
KW   .
XX
OS   Chironomus thummi
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera;
OC   Endopterygota; Diptera; Nematocera; Chironomoidea; Chironomidae;
OC   Chironominae; Chironomus.
XX
RN   [1]
RP   1-3644
RX   MEDLINE; 96199249.
RX   PUBMED; 8621663.
RA   Hoffman R.T., Schmidt E.R., Case S.T.;
RT   "A cell-specific glycosylated silk protein from Chironomus thummi salivary
RT   glands. Cloning, chromosomal localization, and characterization of cDNA";
RL   J. Biol. Chem. 271(16):9809-9815(1996).
XX
RN   [2]
RP   1-3693
RX   MEDLINE; 99077856.
RX   PUBMED; 9858763.
RA   Berezikov E., Blinov A.G., Scherbik S., Cox C.K., Case S.T.;
RT   "Structure and polymorphism of the Chironomus thummi gene encoding special
RT   lobe-specific silk protein, ssp160";
RL   Gene 223(1-2):347-354(1998).
XX
RN   [3]
RP   1-3693
RA   Berezikov E., Blinov A.G., Scherbik S.S., Cox C.C., Case S.T.;
RT   ;
RL   Submitted (05-DEC-1997) to the EMBL/GenBank/DDBJ databases.
RL   Biochemistry, University of Mississippi Medical Center, 2500 North State
RL   Street, Jackson, MS 39216-4505, USA
XX
CC   On Mar 4, 1998 this sequence version replaced gi:2707296.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..3693
FT                   /chromosome="IV"
FT                   /db_xref="taxon:7154"
FT                   /organism="Chironomus thummi"
FT                   /strain="German"
FT                   /map="A2b"
FT                   /dev_stage="larval"
FT                   /cell_type="lobe-specific secretory"
FT                   /tissue_type="salivary gland"
FT   CAAT_signal     84..87
FT                   /gene="ssp160"
FT   TATA_signal     169..172
FT                   /gene="ssp160"
FT   mRNA            join(198..288,604..759,825..2408,2874..3129,3188..3241,
FT                   3299..3569)
FT                   /gene="ssp160"
FT                   /product="special lobe-specific silk protein ssp160"
FT   5'UTR           198..234
FT                   /gene="ssp160"
FT   CDS             join(235..288,604..759,825..2408,2874..3129,3188..3241,
FT                   3299..3441)
FT                   /codon_start=1
FT                   /db_xref="UniProt/TrEMBL:O44416"
FT                   /note="160 kDa secretory protein"
FT                   /gene="ssp160"
FT                   /product="special lobe-specific silk protein ssp160"
FT                   /protein_id="AAD11516.1"
FT                   /translation="MNIKVILVCALVAIFFAQVAEGGPIANFVGFIISLLFSLFEVMLS
FT                   VVFDVKSFTSLSNATANATMPGFASSVGGGRFTVIMKGTFNLIAMISANIQAIQSGSGS
FT                   ASSNSSSSANSTTSSNSTTSSNSTTSSNSTTSSNSTSSGLTTGASVVSLIDTCAWVYQD
FT                   SSVGIAYLMVSILALFYGQSVSAPPYADLGIPALPANTSGAGVPQSVQIKAAITYINVT
FT                   INFITLTGQQFEDLQGPVTTDCGCPNTTSVAPLVAEWEAIMAALEAFASGSASSNSTSN
FT                   STSTSNSTTTSNSTTTTNSTTSTNSTSSSNSTTIAGSIDIAANLTVALQNLQALLMQEA
FT                   TCAPCLAANAKKSGVREFGPCKAAGSSCARSGQRKVKRKARLEKMRAKSRRAVGNRKGS
FT                   MKKRVRSRAKKFGKAAKSGVRRYRKNIKFVYIPPVMASLNAYAALMASLSDSISYQSES
FT                   ALNSTDSACNSTSNSTDEAVINATTAVTDMFVNFTAMVINNTVAHPNCTQYADMALSMV
FT                   SQINEQIIACGSQSDSAQSSIYANVTISIVAMAQEYNNFASMSDKCTRSFANSWLWMYI
FT                   KWVFYRMGMTSGVPNFLACQTKAQSSLTAFLASFNATVSATISAASANNSEVQSSEAAC
FT                   IESSLAEAAVILEMFEAAYQNCTDPGSVTVPAETTTTTSTTTTTTTTTTTAAPTTTTTK
FT                   AANAPFTYPLCTLIMSTTCSLGGAGCTYPLISSAGCCPSGKTLNTGLGGRGCCK"
FT   3'UTR           3442..3569
FT                   /gene="ssp160"
FT   polyA_signal    order(3520..3523,3532..3537,3539..3544)
FT                   /gene="ssp160"
XX
SQ   Sequence 3693 BP; 1171 A; 768 C; 633 G; 1121 T; 0 other;
     aagctttcaa ctctcattta aacctaaata agcacatcat atccacaatt catcagccaa        60
     taaaaattca ctttatttta atccaatcaa agaaagtttc acgatgtcaa aaacattcgt       120
     ggatgtcccg tcaagtggaa ggagaaacga aaattcacga aatttcacaa taaaaagtga       180
     gtaagttgtg tggtccatgt catatcgatc taatttatcg aatagtgata aaatatgaat       240
     attaaagtga tcttagtgtg cgccttggtt gcgatcttct ttgcacaggt aagttggtaa       300
     cttgggttgg gatcttcttt aaatttgact agctttgtac aagatatgag ttgtatagtc       360
     tgtagttgta gtgtcatggg ttgtatactg tcttgtaggc taggttttgc ggctcttgca       420
     caaatatatc ttaaaaagat cttaaaagtc aacgagagtg ttcaataacg tgatcaataa       480
     caagcccatg atacgtctca gctccttaaa tccaccaaat atgactcata tacagacaca       540
     accctaatct taaagtacta agcaatttta atcacaattt taaattttcc acatcttatc       600
     caggttgcag aaggcggacc aatcgccaat tttgtaggct tcatcatctc cctcttgttc       660
     tccttgtttg aagtcatgct gtcagttgtg ttcgatgtca agtcgttcac aagcttgtca       720
     aatgccacag caaatgcaac gatgcctggt tttgcatctg tgagtatttt tagctatagc       780
     ttgacctgca aaatccttga taaatatgtt gattccattt ctagagcgtt ggtggtggtc       840
     gcttcacagt catcatgaaa ggaacattca atttgattgc catgatatcg gcaaatattc       900
     aagccattca gtcaggatca ggatcagcat cgtctaattc ctcatcaagt gctaattcta       960
     caacatcctc aaactcaaca acaagctcaa actcaacgac atcctcgaac tcaacaacaa      1020
     gctcaaactc tacatccagt ggattaacaa ccggtgctag cgttgtaagc ttgattgata      1080
     cctgtgcttg ggtctatcag gacagttcag ttggaattgc ctacttgatg gtctcaattt      1140
     tggcactttt ctatggacaa tctgtctcag caccgccata tgctgatctt ggtataccag      1200
     ctctaccagc aaatacctct ggtgctggag ttccacaatc tgtacaaatt aaagcagcaa      1260
     ttacttacat caatgttact attaacttta ttaccttaac tggtcaacaa tttgaagatt      1320
     tacaaggtcc agttaccaca gattgtggat gtccaaatac aactagtgtt gcgccacttg      1380
     ttgctgaatg ggaagccata atggctgctc ttgaagcttt cgctagtgga tcagcatcat      1440
     ctaattctac atctaattca acatcaacaa gtaactcaac aacgacaagc aactcaacaa      1500
     ctacgacaaa ttcaacaact tcaactaatt caacatcctc gtcaaattcg acaacaattg      1560
     ctggatctat tgacattgct gctaatctta cagttgccct ccagaacctc caagccttgc      1620
     tcatgcaaga agctacttgt gctccatgcc tagctgcaaa tgccaaaaag agtggtgttc      1680
     gtgaatttgg accatgtaaa gctgctggct catcatgtgc tagatcagga caaagaaaag      1740
     tcaagcgtaa ggcacgattg gagaagatgc gtgccaagtc ccgtcgtgca gtcggtaaca      1800
     gaaaaggatc aatgaagaag cgcgtccgta gtcgtgcaaa gaaattcgga aaggctgcaa      1860
     agtcaggagt tagacgatac cgcaagaaca tcaagtttgt ctacattcca ccagtcatgg      1920
     cttcacttaa tgcatatgca gcattgatgg catctttaag tgacagcatc tcatatcaat      1980
     ctgaatcggc tttgaactca acagactctg catgtaactc aacatcaaac agcacagatg      2040
     aggctgtaat taatgcaaca accgctgtta ctgatatgtt tgtcaacttt actgctatgg      2100
     tcatcaataa tactgttgca catccaaatt gtactcaata tgctgacatg gctctttcaa      2160
     tggtctctca aatcaacgag caaatcattg catgcggaag tcagtctgat tctgctcagt      2220
     catcaatcta tgcaaatgtt acaatcagta ttgttgcaat ggctcaagaa tacaataact      2280
     ttgcatctat gtctgataag tgtaccagat catttgccaa tagttggttg tggatgtaca      2340
     ttaagtgggt cttctatcga atgggaatga cttctggagt tcctaacttc ctcgcttgtc      2400
     aaactaaggt ttgtgattta cttaacaaat acttgaaact aaacgtatcc cttagaactg      2460
     aacttatcat tagaaattga acttatcctt taaaaactga acttatcccg agaaaagaaa      2520
     tgtatcctta gaaacgaaat cggttttaaa aactgagctt attcttcagg aactaaactc      2580
     atccttagaa actgaccata tccttaaaaa ctgaacgtat tcttagaagc taactcatta      2640
     ttcagaaact gaacttgacc ttagaaactg aacatattct tcggactaaa ctcatcctaa      2700
     gaaactgacc gtatccttaa aaactgaact tagaagctga actcattatt cagaaactga      2760
     agttcaccct tagaaactga acttattctt caggaactaa acttatcctt tgaaactgaa      2820
     cttatcctta aaaactgatt ttactgagtc cttctttaat ccttcattaa taggctcaaa      2880
     gcagtcttac agcattcttg gcatcattta atgccacagt cagtgccacg atttcagcag      2940
     caagtgcaaa taattctgaa gttcaaagct ctgaagcagc ttgtattgaa agtagtcttg      3000
     cggaagcagc cgttatttta gaaatgtttg aggcagcata tcaaaactgt acagatccag      3060
     gatccgtaac agttccagca gagactacaa caacgaccag tacaaccacc actacaacta      3120
     ccacaacaag taggttaaaa ttggaaaagt ttagaatttt gtgtgcaatt tttttaattt      3180
     tttacagcaa ccgcagcacc aacaacaacc acaactaagg ctgctaatgc accattcaca      3240
     tgtaagatat gaaatcaaat tgtgactttt tctaagaaat tgtttccttt ttttctagat      3300
     ccattgtgta ctttgatcat gtcaacaaca tgctcactgg gtggagcagg atgtacatat      3360
     ccacttatct catctgctgg ctgctgtcca tctggcaaaa cattaaacac tggtctcggt      3420
     ggacgtggtt gttgcaaata aaatctacag cgacagttaa attgaagcaa ttattttttc      3480
     ctatttcttg atgtagaaca ttttttgtac ttaattttga taaaatgtgg cattaaacat      3540
     ataatctgaa atgtggacga gaaactttat tttaaactat ttatttattt tttaatcttt      3600
     tcagaaactc aatttatccc ataggaactg aacttatcct taataaactg gtcttatctt      3660
     aagcaacaaa acgttttctt ttcaactgaa ttc                                   3693
//
