Entry information
Entry name | VE6_HPV16
Primary accession | P03126
Secondary accessions | Q71BI7
integrated into UniProtKB/Swiss-Prot | 21 July 1986
sequence version 1 | 21 July 1986
entry version 76 | 23 March 2010
Name and origin of the protein
Protein name | Protein E6
Gene names |
Name | E6
From | Human papillomavirus type 16 (Taxon ID: 333760)
Taxonomy | Viruses; dsDNA viruses, no RNA stage; Papillomaviridae; Alphapapillomavirus
Keywords | 3D-structure; Activator; DNA-binding; Early protein; Host nucleus; Host-virus interaction; Metal-binding; Oncogene; Transcription; Transcription regulation; Zinc; Zinc-finger
References
1 | Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W.G.; "Human papillomavirus type 16 DNA sequence." Virology 145:181-185(1985)
MEDLINE | 85246220
PubMed | 2990099
DOI | 10.1016/0042-6822(85)90214-4
Reference Position | nucleotide sequence [genomic dna].
2 | Terai M., Fu L., Ma Z., Burk R.D.; "Cloning and sequencing of non-European human papillomavirus (HPV) variant complete genomes from cervicovaginal cells by an overlapping PCR method." Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Reference Position | nucleotide sequence [genomic dna].
Reference Comment | strain=isolate european german 131
3 | Schneider-Maunoury S., Pehau-Arnaudet G., Breitburd F., Orth G.; "Expression of the human papillomavirus type 16 genome in SK-v cells, a line derived from a vulvar intraepithelial neoplasia." J. Gen. Virol. 71:809-817(1990)
MEDLINE | 90218027
PubMed | 2157796
Reference Position | nucleotide sequence [genomic dna] of 31-50.
4 | Du M., Fan X., Hong E., Chen J.J.; "Interaction of oncogenic papillomavirus E6 proteins with fibulin-1." Biochem. Biophys. Res. Commun. 296:962-969(2002)
MEDLINE | 22188366
PubMed | 12200142
DOI | 10.1016/S0006-291X(02)02041-7
Reference Position | interaction with human fbln1, and inhibition of e6-mediated transformation.
5 | Gewin L., Myers H., Kiyono T., Galloway D.A.; "Identification of a novel telomerase repressor that interacts with the human papillomavirus type-16 E6/E6-AP complex." Genes Dev. 18:2269-2282(2004)
PubMed | 15371341
DOI | 10.1101/gad.1214704
Reference Position | interaction with human nfx1.
6 | Thomas M., Laura R., Hepner K., Guccione E., Sawyers C., Lasky L., Banks L.; "Oncogenic human papillomavirus E6 proteins target the MAGI-2 and MAGI-3 proteins for degradation." Oncogene 21:5088-5096(2002)
PubMed | 12140759
DOI | 10.1038/sj.onc.1205668
Reference Position | interaction with human magi3.
7 | Lee C., Laimins L.A.; "Role of the PDZ domain-binding motif of the oncoprotein E6 in the pathogenesis of human papillomavirus type 31." J. Virol. 78:12366-12377(2004)
PubMed | 15507623
DOI | 10.1128/JVI.78.22.12366-12377.2004
Reference Position | interaction with human magi3.
8 | Massimi P., Gammoh N., Thomas M., Banks L.; "HPV E6 specifically targets different cellular pools of its PDZ domain-containing tumour suppressor substrates for proteasome-mediated degradation." Oncogene 23:8033-8039(2004)
PubMed | 15378012
DOI | 10.1038/sj.onc.1207977
Reference Position | interaction with human magi3.
Comments
function | Transcriptional transactivator. Binds double stranded DNA. Has transforming activity. Inactivates, with E6-AP ubiquitin- protein ligase, the human TP53/p53 tumor suppressor protein by targeting it to degradation. Binds and targets human MUPP1/MPDZ protein to degradation. Those two functions presumably contribute to transforming activity (By similarity). Interaction with human FBLN1 protein also seems to be linked to cell transformation.
subunit | Forms a complex with E6-AP ubiquitin-protein ligase which interacts with human P53. Binds to human FBLN1 and MPDZ (By similarity). Interacts with human NFX1 and MAGI3.
interaction | O15350-1:TP73 (xeno); NbExp=1; IntAct=EBI-1177242, EBI-389619; O15350-2:TP73 (xeno); NbExp=1; IntAct=EBI-1177242, EBI-389623;
subcellular location | Host nucleus matrix.
miscellaneous | HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans.
similarity | Belongs to the papillomaviridae E6 protein family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL | K02718; AAA46939.1; -; Genomic_DNA.
AF536179; AAQ10712.1; -; Genomic_DNA.
D00735; BAA00632.1; -; Genomic_DNA.
PIR | A03682; W6WLHS.
RefSeq | NP_041325.1; -.
PDB | 1VZN; Model; -; A=1-158.
2FK4; NMR; -; A=88-158.
PDBsum | 1VZN; -.
2FK4; -.
IntAct | P03126; 5.
MINT | MINT-96563; -.
GeneID | 1489078; -.
GO | ; C:host cell nuclear matrix; IEA:UniProtKB-SubCell.
; F:DNA binding; IEA:UniProtKB-KW.
; F:protein binding; IPI:IntAct.
; F:zinc ion binding; IEA:UniProtKB-KW.
; P:cellular transcription; IEA:UniProtKB-KW.
; P:interspecies interaction between organisms; IEA:UniProtKB-KW.
; P:regulation of cellular transcription; IEA:UniProtKB-KW.
InterPro | IPR001334; E6.
Pfam | PF00518; E6; 1.
Features
Key | From | To | Length | Description
--- | --- | --- | --- | ---
chain | 1 | 158 | 158 | Protein E6. /FTId=PRO_0000133336.
zn_fing | 37 | 73 | 37 | Potential.
zn_fing | 110 | 146 | 37 | Potential.
motif | 155 | 158 | 4 | PDZ-binding (By similarity).
conflict | 17 | 17 | 1 | R -> G (in Ref. 2; AAQ10712).
conflict | 90 | 90 | 1 | L -> V (in Ref. 2; AAQ10712).
strand | 87 | 90 | 4 |
helix | 93 | 100 | 8 |
turn | 101 | 105 | 5 |
strand | 111 | 113 | 3 |
helix | 119 | 128 | 10 |
strand | 132 | 135 | 4 |
strand | 138 | 141 | 4 |
helix | 142 | 146 | 5 |
Sequence information
Length: 158 aa, molecular weight 19187 Da, CRC64 checksum 01FEF5ADCFDB37EB
    MHQKRTAMFQ DPQERPRKLP QLCTELQTTI HDIILECVYC KQQLLRREVY DFAFRDLCIV
    YRDGNPYAVC DKCLKFYSKI SEYRHYCYSL YGTTLEQQYN KPLCDLLIRC INCQKPLCPE
    EKQRHLDKKQ RFHNIRGRWT GRCMSCCRSS RTRRETQL