digraph g
{
	graph
	[
		rankdir = "LR"
	]
	node
	[
		shape = "rpromoter"
		colorscheme = "rdbu5"
		color = "1"
		style = "filled"
		fontcolor = "3"
	]
	Hef1a
	TRE
	UAS
	Hef1aLacOid
	Hef1aLacOid
	[
		label = "Hef1a-LacOid"
	]
	node
	[
		shape = "rarrow"
		colorscheme = "rdbu5"
		color = "5"
		style = "filled"
		fontcolor = "3"
	]
	Gal4VP16
	LacI
	rtTA3
	DeltamCherry
	Gal4VP16
	[
		label = "Gal4-VP16"
	]
	product
	[
		shape = "oval"
		style = "filled"
		colorscheme = "rdbu5"
		color = "2"
		label = ""
	]
	repression
	[
		shape = "oval"
		label = "LacI repression"
		fontcolor = "black"
		style = "dotted"
	]
	node
	[
		shape = "oval"
		style = "filled"
		colorscheme = "rdbu5"
		color = "4"
		fontcolor = "5"
	]
	combination
	[
		label = "rtTA3 + Doxycycline"
	]
	LacIprotein
	[
		label = "LacI"
	]
	rtTA3protein
	[
		label = "rtTA3"
	]
	Gal4VP16protein
	[
		label = "Gal4-VP16"
	]
	subgraph cluster_0
	{
		graph
		[
			colorscheme = "rdbu5"
		]
		graph
		[
			color = "3"
		]
		node
		[
			colorscheme = "rdbu5"
			fontcolor = "3"
		]
		Hef1a -> Gal4VP16
		[
			arrowhead = "none"
		]
		Gal4VP16 -> UAS
		[
			arrowhead = "none"
		]
		UAS -> LacI
		[
			arrowhead = "none"
		]
		LacI -> Hef1aLacOid
		[
			arrowhead = "none"
		]
		Hef1aLacOid -> rtTA3
		[
			arrowhead = "none"
		]
		rtTA3 -> TRE
		[
			arrowhead = "none"
		]
		TRE -> DeltamCherry
		[
			arrowhead = "none"
		]
	}
	Gal4VP16 -> Gal4VP16protein
	Gal4VP16protein -> UAS
	LacI -> LacIprotein
	LacIprotein -> repression
	repression -> Hef1aLacOid
	[
		arrowhead = "tee"
	]
	IPTG -> repression
	[
		arrowhead = "tee"
	]
	rtTA3 -> rtTA3protein
	rtTA3protein -> combination
	combination -> TRE
	Doxycycline -> combination
	DeltamCherry -> product
}
